赣南医科大学学报

目的：筛选芒果苷脂质体的制备方法及最佳处方。方法：以平均粒径、聚合物分散性指数(Polydispersity index,PDI)、Zeta电位为考察指标筛选，通过L₉(3⁴)正交实验，确定芒果苷脂质体的最佳处方及制备方法。再对筛选出的最佳处方及制备方法制备出的芒果苷脂质体成品进行表征，使用高效液相色谱仪测定其包封率。结果：最佳处方：DOTAP∶胆固醇∶葵花磷脂∶芒果苷=2∶6∶1.5∶1(摩尔比)，使用薄膜水合法对该脂质体制备效果最佳。成品的平均粒径为(182.20±2.25) nm,PDI为(0.148±0.001),Zeta电位为(45.70±0.56) mV。扫描电镜下芒果苷脂质体形态近圆。包封率为93.85%。结论：制备的芒果苷脂质体平均粒径较小，脂质体体系分布均匀稳定，形态近圆，符合脂质体的各项标准。

Objective : To screen the preparation method and the optimal prescription of mangiferin liposomes. Methods : The average particle size, polydispersity index(PDI), and Zeta potential were used as evaluation indicators. The L₉(3⁴) orthogonal experiment was conducted to determine the optimal prescription and preparation method of mangiferin liposomes. The prepared mangiferin liposomes with the optimal prescription and preparation method were characterized, and the entrapment efficiency was determined by high performance liquid chromatography. Results : The optimal prescription was DOTAP∶ cholesterol∶ sunflower phospholipid∶ mangiferin = 2∶6∶1. 5∶1(molar ratio), and the film hydration method was the best for the preparation of the liposomes. The average particle size of the product was(182. 20±2. 25) nm, the PDI was(0. 148±0. 001), and the Zeta potential was(45. 70±0. 56) mV. The morphology of the mangiferin liposomes was nearly spherical under scanning electron microscopy. The entrapment efficiency was 93. 85%. Conclusion : The prepared mangiferin liposomes had a small average particle size, a uniform and stable liposome system, and a nearly spherical morphology, which met the standards of liposomes.